Eumorphia

Hutchinson-Gilford progeria syndrome (HGPS) is a uncommon accelerated ageing dysfunction most notably characterised by heart problems and untimely demise from myocardial infarction or stroke.

The vast majority of circumstances are attributable to a de novo single nucleotide mutation within the LMNA gene that prompts a cryptic splice donor web site, leading to manufacturing of a poisonous type of lamin A with a 50 amino acid inner deletion, termed progerin.

We beforehand reported the era of a transgenic murine mannequin of progeria carrying a human BAC harboring the frequent mutation, G608G, which within the single-copy state develops options of HGPS which might be restricted to the vascular system. Right here, we report the phenotype of mice bred to hold two copies of the BAC, which extra utterly recapitulate the phenotypic options of HGPS in pores and skin, adipose, skeletal, and vascular tissues.

We additional present that genetic discount of the mechanistic goal of rapamycin (mTOR) considerably extends lifespan in these mice, offering a rationale for pharmacologic inhibition of the mTOR pathway within the therapy of HGPS.

Guanidinoacetate methyltransferase deficiency (GAMT-D) is one in every of three cerebral creatine (Cr) deficiency syndromes attributable to pathogenic variants within the GAMT gene (19p13.3). GAMT-D is characterised by the buildup of guanidinoacetic acid (GAA) and the depletion of Cr, which end in extreme world developmental delay (and mental incapacity), motion dysfunction, and epilepsy.

The GAMT knockout (KO) mouse mannequin presents biochemical alterations in bodily fluids, the mind, and muscle tissue, together with elevated GAA and decreased Cr and creatinine (Crn) ranges, that are just like these noticed in people.

On the behavioral stage, solely restricted and gentle alterations have been reported, with a big a part of analyzed behaviors being unaffected in GAMT KO as in contrast with wild-type mice. On the cerebral stage, decreased Cr and Crn and elevated GAA and different guanidine compound ranges have been noticed. However, the consequences of Cr deficiency and GAA accumulation on many neurochemical, morphological, and molecular processes haven’t but been explored.

On this evaluate, we summarize information concerning behavioral and cerebral GAMT KO phenotypes, and deal with uncharted behavioral alterations which might be comparable with the medical signs reported in GAMT-D sufferers, together with mental incapacity, poor speech, and autistic-like behaviors, in addition to unexplored Cr-induced cerebral alterations.

We now have been utilizing the Inbred Lengthy- and Quick-Sleep mouse strains (ILS, ISS) and a recombinant inbred panel derived from them, the LXS, to research the genetic underpinnings of acute ethanol tolerance which is taken into account to be a threat issue for alcohol use issues (AUDs).

Right here, we’ve got used RNA-seq to look at the transcriptome of complete mind in 40 of the LXS strains eight hours after a saline or ethanol “pretreatment” as in earlier behavioral research. Roughly 1/Three of the 14,184 expressed genes had been considerably heritable and lots of had been distinctive to the pretreatment.

A number of thousand cis- and trans-eQTLs had been mapped; a portion of those additionally had been distinctive to pretreatment. Ethanol pretreatment triggered differential expression (DE) of 1,230 genes. Gene Ontology (GO) enrichment evaluation advised involvement in quite a few organic processes together with astrocyte differentiation, histone acetylation, mRNA splicing, and neuron projection growth.

Genetic correlation evaluation recognized a whole bunch of genes that had been correlated to the behaviors. GO evaluation indicated that these genes are concerned in gene expression, chromosome group, and protein transport, amongst others.

The expression profiles of the DE genes and genes correlated to AFT within the ethanol pretreatment group (AFT-Et) had been discovered to be just like profiles of HDAC inhibitors. Hdac1, a cis-regulated gene that’s situated on the peak of a beforehand mapped QTL for AFT-Et, was correlated to 437 genes, most of which had been additionally correlated to AFT-Et.

GO evaluation of those genes recognized a number of enriched organic course of phrases together with neuron-neuron synaptic transmission and potassium transport. In abstract, the outcomes recommend widespread genetic results on gene expression, together with results which might be pretreatment-specific.

Quite a lot of candidate genes and organic capabilities had been recognized that may very well be mediating the behavioral responses. Probably the most outstanding of those was Hdac1 which can be regulating genes related to glutamatergic signaling and potassium conductance.

CRISPR/Cas9 equipment delivered as ribonucleoprotein (RNP) to the zygote has grow to be a normal software for the event of genetically modified mouse fashions. In recent times, numerous experiences have demonstrated the efficient supply of CRISPR/Cas9 equipment through zygote electroporation as an alternative choice to the standard supply methodology of microinjection.

On this examine, we’ve got carried out side-by-side comparisons of the 2 RNP supply strategies throughout a number of gene loci and conclude that electroporation compares very favourably with typical pronuclear microinjection, and report an enchancment in mutagenesis effectivity when delivering CRISPR through electroporation for the era of straightforward knock-in alleles utilizing single-stranded oligodeoxynucleotide (ssODN) restore templates.

As well as, we present that the effectivity of knock-in mutagenesis could be additional elevated by electroporation of embryos derived from Cas9-expressing donor females. The maternal provide of Cas9 to the zygote avoids the need to ship the comparatively massive Cas9 protein, and excessive effectivity era of each indel and knock-in allele could be achieved by electroporation of small single-guide RNAs and ssODN restore templates alone.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-48T	DL Develop	48T	EUR 380
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-96T	DL Develop	96T	EUR 485
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-48T	DL Develop	48T	EUR 547
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-96T	DL Develop	96T	EUR 715
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-48T	DL Develop	48T	EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-48Tests	Reddot Biotech	48 Tests	EUR 580

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-96Tests	Reddot Biotech	96 Tests	EUR 807

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-48Tests	Reddot Biotech	48 Tests	EUR 557

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-96Tests	Reddot Biotech	96 Tests	EUR 774

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 383

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 525

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-48Tests	Reddot Biotech	48 Tests	EUR 580

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-96Tests	Reddot Biotech	96 Tests	EUR 807

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 534

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 742

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-48Tests	Reddot Biotech	48 Tests	EUR 555

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-96Tests	Reddot Biotech	96 Tests	EUR 771

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-48Tests	Reddot Biotech	48 Tests	EUR 533

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-96Tests	Reddot Biotech	96 Tests	EUR 740

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 367

Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 502

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-48Tests	Reddot Biotech	48 Tests	EUR 555

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-96Tests	Reddot Biotech	96 Tests	EUR 771

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 511

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 709

Anti-LIF Antibody
PA1562	BosterBio	100ug/vial	EUR 294

Anti-LIF Antibody
PB9036	BosterBio	100ug/vial	EUR 334

Anti-LIF Antibody
A01400	BosterBio	100ug/vial	EUR 294

Anti-LIF antibody
STJ96472	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to LIF.

Anti-LIF antibody
STJ73631	St John's Laboratory	100 µg	EUR 359

Anti-LIF antibody
STJ29846	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

anti- LIF antibody
FNab09804	FN Test	100µg	EUR 548.75
Description: Antibody raised against LIF

anti- LIF antibody
LSMab09804	Lifescience Market	100 ug	EUR 386

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

LIF, Mouse
HY-P7084	MedChemExpress	50ug	EUR 533

LIF, mouse
MO16102	Neuromics	100 ug	EUR 675

Anti-LIF (aa28-39) antibody
STJ72919	St John's Laboratory	100 µg	EUR 359

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280

Mouse LIF ELISA Kit
EML0014	Abclonal	96Tests	EUR 521

Mouse LIF shRNA Plasmid
20-abx971321	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Recombinant Mouse LIF Protein
R01400-1	BosterBio	100ug/vial	EUR 294

LIF, Human
HY-P7049	MedChemExpress	10ug	EUR 1600

LIF siRNA
20-abx922546	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

LIF siRNA
20-abx922547	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

LIF Antibody
25283-100ul	SAB	100ul	EUR 390

LIF Antibody
36950-100ul	SAB	100ul	EUR 252

LIF, human
MO16101	Neuromics	100 ug	EUR 688

LIF Antibody
1-CSB-PA198481	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody
1-CSB-PA012928ESR2HU	Cusabio	EUR 222.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF Antibody
1-CSB-PA004761	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

rHu LIF
AK9977-0005	Akron Biotech	5µg	Ask for price

rHu LIF
AK9977-0025	Akron Biotech	25µg	Ask for price

rHu LIF
AK9977-0100	Akron Biotech	100µg	Ask for price

rHu LIF
AK9977-1000	Akron Biotech	1mg	Ask for price

LIF Antibody
5216-100	Biovision		EUR 316

LIF Antibody
5216-30T	Biovision		EUR 146

LIF antibody
PAab09804	Lifescience Market	100 ug	EUR 386

LIF antibody
70R-6230	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal LIF antibody

LIF protein
30R-2771	Fitzgerald	10 ug	EUR 369
Description: Purified recombinant Human LIF protein

LIF protein
30R-2772	Fitzgerald	10 ug	EUR 489
Description: Purified recombinant Mouse LIF protein

LIF antibody
70R-12299	Fitzgerald	100 ug	EUR 403
Description: Rabbit polyclonal LIF antibody

LIF antibody
70R-14100	Fitzgerald	100 ug	EUR 322
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF antibody
70R-51295	Fitzgerald	100 ul	EUR 244
Description: Purified Polyclonal LIF antibody

LIF Peptide
6245P	ProSci	0.05 mg	EUR 164.75
Description: (IN) LIF peptide

LIF Peptide
43-200P	ProSci	0.1 mg	EUR 338

LIF Antibody
6245-002mg	ProSci	0.02 mg	EUR 171.82
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody
6245-01mg	ProSci	0.1 mg	EUR 436.42
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

Mouse LIF PicoKine ELISA Kit
EK0580	BosterBio	96 wells	EUR 425
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

LIF ELISA Kit (Mouse) (OKBB00760)
OKBB00760	Aviva Systems Biology	96 Wells	EUR 505
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

LIF ELISA Kit (Mouse) (OKEH04045)
OKEH04045	Aviva Systems Biology	96 Wells	EUR 544
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

ELISA kit for Mouse LIF
EK5250	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

LIF, Mouse Recombinant , 1MIU/ml
L4500-001	GenDepot	1ml	EUR 339

LIF, Mouse Recombinant , 1MIU/ml
L4500-005	GenDepot	5x1ml	EUR 1193

LIF, Mouse Recombinant , 10MIU/ml
L4501-001	GenDepot	1ml	EUR 1454

Leukemia Inhibitory Factor (LIF), mouse
PR16102	Neuromics	5 ug	EUR 435

Human CellExp? LIF, mouse recombinant
7268-10	Biovision		EUR 370

Lif ORF Vector (Mouse) (pORF)
ORF049145	ABM	1.0 ug DNA	EUR 506

Lif ORF Vector (Mouse) (pORF)
ORF049146	ABM	1.0 ug DNA	EUR 506

LIF Polyclonal Antibody
ES3837-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

LIF Polyclonal Antibody
ES3837-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

LIF Polyclonal Antibody
ABP52838-003ml	Abbkine	0.03ml	EUR 158
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF

LIF Polyclonal Antibody
ABP52838-01ml	Abbkine	0.1ml	EUR 289
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF

LIF Polyclonal Antibody
ABP52838-02ml	Abbkine	0.2ml	EUR 414
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF

LIF Blocking Peptide
20-abx064170	Abbexa	EUR 272.00 EUR 411.00	1 mg 5 mg

LIF Rabbit pAb
A1288-100ul	Abclonal	100 ul	EUR 308

LIF Rabbit pAb
A1288-200ul	Abclonal	200 ul	EUR 459

Moreover, electroporation, in comparison with microinjection, leads to the next price of embryo survival and growth. The tactic thus has the potential to cut back the variety of animals used within the manufacturing of genetically modified mouse fashions.