Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations

Here, we describe the Control of Lineages by Barcode Enabled Recombinant¬†Transcription (COLBERT) method in which unique single guide RNA (sgRNA) barcodes are used as functional¬†tags¬†to identify and recall specific lineages of interest. An sgRNA barcode is stably integrated and actively transcribed, such that all cellular progeny will contain the parental barcode and produce a functional…

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