Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations

Here, we describe the Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT) method in which unique single guide RNA (sgRNA) barcodes are used as functional tags to identify and recall specific lineages of interest. An sgRNA barcode is stably integrated and actively transcribed, such that all cellular progeny will contain the parental barcode and produce a functional…

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Saliva test for COVID-19

Abstract Background Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate…

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Long-term changes in Al thin-film extreme ultraviolet filters

Long-term changes in Al thin-film extreme ultraviolet filters

Skinny-film Al filters are very fashionable owing to their excessive transmittance within the wavelength vary of 17-67 nm and low transmittance within the seen and near-UV areas; nonetheless, they’re susceptible to oxidation. The amorphous Al2O3 layers on the Al surfaces have a lot smaller transmittance than the majority Al materials; due to this fact, they strongly affect…

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Research on the lossless contention MAC protocol and the performance of an ultraviolet communication network

Research on the lossless contention MAC protocol and the performance of an ultraviolet communication network

Ultraviolet (UV) communication has been the subject of intensive current analysis resulting from its non-line-of-sight (NLOS) communication, anti-interference, and excessive confidentiality. Given the dearth of the UV Media Entry Management (MAC) protocol, this paper proposes a UV lossless rivalry MAC (UVLLC-MAC) protocol creatively. This MAC protocol is predicated on the superposition logic of UV energy,…

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