Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations

Here, we describe the Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT) method in which unique single guide RNA (sgRNA) barcodes are used as functional tags to identify and recall specific lineages of interest. An sgRNA barcode is stably integrated and actively transcribed, such that all cellular progeny will contain the parental barcode and produce a functional…

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Saliva test for COVID-19

Abstract Background Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate…

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Panbio Nasopharyngeal Test

COVID-19 Ag Rapid Test Device

Abstract Several rapid testing methodologies have been approved for testing of symptomatic individuals but have not been validated for asymptomatic screening. We evaluated performance of the Abbott PanbioTM COVID-19 rapid antigen assay in the asymptomatic setting. We conducted a prospective study in an urban assessment center and in the context of long-term care staff screening.…

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Long-term changes in Al thin-film extreme ultraviolet filters

Long-term changes in Al thin-film extreme ultraviolet filters

Skinny-film Al filters are very fashionable owing to their excessive transmittance within the wavelength vary of 17-67 nm and low transmittance within the seen and near-UV areas; nonetheless, they’re susceptible to oxidation. The amorphous Al2O3 layers on the Al surfaces have a lot smaller transmittance than the majority Al materials; due to this fact, they strongly affect…

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Research on the lossless contention MAC protocol and the performance of an ultraviolet communication network

Research on the lossless contention MAC protocol and the performance of an ultraviolet communication network

Ultraviolet (UV) communication has been the subject of intensive current analysis resulting from its non-line-of-sight (NLOS) communication, anti-interference, and excessive confidentiality. Given the dearth of the UV Media Entry Management (MAC) protocol, this paper proposes a UV lossless rivalry MAC (UVLLC-MAC) protocol creatively. This MAC protocol is predicated on the superposition logic of UV energy,…

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Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

Deep-ultraviolet laser ablation with a pulsed 193 nm ArF excimer laser was used to take away localized areas from tissue sections from which proteins have been extracted for spatially resolved proteomic evaluation by liquid chromatography tandem mass spectrometry (LC-MS/MS). The power to seize intact proteins by ablation at 193 nm wavelength was verified by matrix-assisted…

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Impact of ultraviolet radiation on the retina

Impact of ultraviolet radiation on the retina

Mild has an important function within the visible course of. For nonvisible radiation within the short-wave spectrum, there are pure mechanisms that shield the human retina from damaging ultraviolet (UV) radiation. Right here, the dose (= power) makes the poison. Injury attributable to UV mild primarily impacts the outer retina, notably the photoreceptors and the…

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Basic Amino Acid-Modified Lignin-Based Biomass Adjuvants: Synthesis, Emulsifying Activity, Ultraviolet Protection, and Controlled Release of Avermectin

Basic Amino Acid-Modified Lignin-Based Biomass Adjuvants: Synthesis, Emulsifying Activity, Ultraviolet Protection, and Controlled Release of Avermectin

Avermectin (AVM) is a extremely efficient and protected biopesticide however may be very delicate to ultraviolet (UV) gentle and displays poor water solubility. Creating inexperienced and multifunctional adjuvants is necessary for the safety and managed launch of AVM. On this work, a lot of water-soluble enzymatic hydrolysis lignins (W-EHLs) have been ready by way of…

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Genetic and functional evidence for gp130/IL6ST-induced TRPA1 upregulation in uninjured but not injured neurons in a mouse model of neuropathic pain

Genetic and functional evidence for gp130/IL6ST-induced TRPA1 upregulation in uninjured but not injured neurons in a mouse model of neuropathic pain

Peripheral nerve accidents end in pronounced alterations in dorsal root ganglia (DRG), which might result in the event of neuropathic ache. Though the polymodal mechanosensitive transient receptor potential ankyrin 1 ion channel (TRPA1) is rising as a related goal for potential analgesic therapies, preclinical research don’t present unequivocal mechanistic perception into its relevance for neuropathic…

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Towards Post-Meiotic Sperm Production: Genetic Insight into Human Infertility from Mouse Models

Towards Post-Meiotic Sperm Production: Genetic Insight into Human Infertility from Mouse Models

Declined high quality and amount of sperm is presently the main reason for sufferers affected by infertility. Male germ cell growth is spatiotemporally regulated all through the entire developmental course of. Whereas it has been identified that exogenous components, similar to environmental publicity, eating regimen and life-style, et al, play causative roles in male infertility,…

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