Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

Deep-ultraviolet laser ablation with a pulsed 193 nm ArF excimer laser was used to take away localized areas from tissue sections from which proteins have been extracted for spatially resolved proteomic evaluation by liquid chromatography tandem mass spectrometry (LC-MS/MS). The power to seize intact proteins by ablation at 193 nm wavelength was verified by matrix-assisted…

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The role of genotype and diet in shaping gut microbiome in a genetic Vitamin A deficient mouse model

The role of genotype and diet in shaping gut microbiome in a genetic Vitamin A deficient mouse model

Multi-factors have been reported to have an effect on the intestine microbiome, together with genotype, age, eating regimen, and diet. Nevertheless, few experiences have investigated the relative capability of various elements to form the intestine microbiome in a single research. Our design used a genetic Vitamin A poor mouse mannequin, the Rbp4-/-¬†mouse, feeding with the…

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